amarok says my most played track is Helium by Skyclad. I have played it 3 times since I formatted my hard drive the other day.
It is my 'most played' because I love the chorus, and is one of the few Skyclad songs that I don't feel like I've overplayed too much in the past.
last.fm says my most played track is Big Dipper by Built to Spill, with 31 plays, because, I was pretty obsessed with the entire record over last summer. I've not actually listened to it in a while, I guess I should, and see if it's as good as I remember.
Incidentally, I'm pretty pleased that while Skyclad are by far my most played artist overall (593 plays to Built to Spill's 266) the most played Skyclad track on last.fm is only my 27th most played track overall.